Laser in der Medizin / Laser in Medicine PDF

Microtomy is a method for laser in der Medizin / Laser in Medicine PDF preparation of thin sections for materials such as bones, minerals and teeth, and an alternative to electropolishing and ion milling. A diagram of a microtome drawn by Cummings in 1770. In the beginnings of light microscope development, sections from plants and animals were manually prepared using razor blades.


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Anwendungen von Laserstrahlung in der Medizin sollen unmittelbar der Gesundheit der Menschen zugute kommen. Die Erarbeitung von Laserverfahren setzt ein Zusammenwirken von Medizinern, Physikern und Technikern voraus. Seit 1973 führt die Kongreß-Messe LASER alle 2 Jahre die internationalen Experten in München zusammen. Im Juni 1997 fanden der 13. Internationale Kongress Lasermedizin und die 11. Tagung der deutschen Gesellschaft für Lasermedizin auf der LASER statt. Die Ergebnisse spiegeln eine aktuelle Standortbestimmung der Lasermedizin wider und vermitteln Perspektiven und Impulse für zukünftige Entwicklungen. Der Inhalt der Vorträge wird daher im vorliegenden Band der Fachwelt zugänglich gemacht.

It was found that to observe the structure of the specimen under observation it was important to make clean reproducible cuts on the order of 100 µm, through which light can be transmitted. This allowed for the observation of samples using light microscopes in a transmission mode. One of the first devices for the preparation of such cuts was invented in 1770 by George Adams, Jr. The device was hand operated, and the sample held in a cylinder and sections created from the top of the sample using a hand crank. In 1835, Andrew Prichard developed a table based model which allowed for the vibration to be isolated by affixing the device to the table, separating the operator from the knife. Occasionally, attribution for the invention of the microtome is given to the anatomist Wilhelm His, Sr. The apparatus has enabled a precision in work by which I can achieve sections that by hand I cannot possibly create.

Namely it has enabled the possibility of achieving unbroken sections of objects in the course of research. Other sources further attribute the development to a Czech physiologist Jan Evangelista Purkyně. Several sources describe the Purkyne model as the first in practical use. The obscurities in the origins of the microtome are due to the fact that the first microtomes were simply cutting apparatuses, and the developmental phase of early devices is widely undocumented. At the end of the 1800s, the development of very thin and consistently thin samples by microtomy, together with the selective staining of important cell components or molecules allowed for the visualisation of microscope details. Today, the majority of microtomes are a knife-block design with a changeable knife, a specimen holder and an advancement mechanism.